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After incubation 5 µl from each wells were inoculated on agar medium plates and the plates were incubated for 24 h.
The bacteria were sterilely inoculated on agar slants for 18 hours and then resuspended to a concentration of 1010cfu/ml based on optical density at 550 nm.
Briefly, the strains were inoculated on agar medium containing sucrose as sole carbon source and incubated aerobically at 30 °C for 48 h.
For this purpose, juvenile isolates of the wild-type strain s and of a PaMth1 deletion strain were inoculated on agar plates containing medium with different amounts of hydrogen peroxide and copper sulphate, respectively.
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In both phases, bacterial strains were inoculated on agar-blood in the adapted Petri dishes, at a concentration of 10 bacteria/dish.
Each strain was inoculated on agar-blood in four Petri dishes, and the colony forming units (CFUs) were counted at 24 h and 48 h following the treatment applied to each group.
In this phase, Escherichia coli – ATCC 35218, Staphylococcus aureus susceptible to oxacillin – ATCC 25923, and Pseudomonas aeruginosa susceptible to imipenem and meropenem – ATCC 27853 were inoculated on agar-blood in Petri dishes and incubated aerobically at 35±2°C for 18 to 24 hours.
The bacterial suspension, in a concentration equal to 105 – 106 cfu/ml, was inoculated on Mueller Hilton agar plates, supplemented with 5% sheep blood agar, and the E- test strips were applied.
External ocular specimens were collected using sterile swabs and inoculated on MacConkey agar, Chocolate agar and Blood agar culture Medias.
Material from rectal and pharyngeal swabs was inoculated on cetrimide agar (Cetrimide Agar Base 285420, Becton, Dickinson and Company, Heidelberg, Germany).
Suspected colonies of E. coli (pinkish color appearance on MacConkey agar and metallic sheen on EMB) (Figs. 1 and 2) were then sub-cultured onto blood agar to appreciate colony characteristics and then pure colonies taken from blood agar were inoculated on nutrient agar (OXOID) (non-selective media).
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CEO of Professional Science Editing for Scientists @ prosciediting.com