Exact(21)
OD600 of 0.4 was set as the limit of growth and corresponded to twice the value of the OD of freshly inoculated medium.
Inoculated medium showing an opaque halo around the zone of growth was considered positive.
Inoculated medium was incubated at 30 °C while being shaken at 225 rpm to an OD600 of 2.0.
The initial OD at 600 nm of the inoculated medium for each deletion strain culture was adjusted to the same level and inoculated onto the SC medium with a final HMF concentration of 15 mM.
Each inoculated medium was incubated at agitation speed 150 rpm at 37 °C for overnight.
The inoculated medium was incubated at 30 °C and 200 r/min in the dark for 5 days.
Similar(39)
HepG2/C3A cells were suspended in inoculating medium with 4 mM glutamine and 1X penicillin/streptomycin, seeded at 2500 cells per well into six PM-M1 microplates, and incubated at 37 C with CO2.
Cells of strain TCE1 were inoculated into medium with or without 100 μM CF and their growth was monitored.
Four wells in each plate were not inoculated (LB medium only) for negative control references.
Controls were inoculated with medium only.
Whole stool and inoculated transport medium were stored at 2 8°C for up to 3 days before transport to LNSP.
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