As the government begins its program to inoculate half a million troops against smallpox, it is engaged in a delicate balancing act between the military's need for discipline and efficiency and its obligation to protect vulnerable recruits from an unusually risky vaccine.
Seeds of Glycine soja (50109) were inoculated in half-strength solid MS medium (pH5.8) in the dark until germination.
Briefly, pre-cultivated cells containing approximately 40% lipid component were inoculated in half-strength Guillard's "f" medium (f/2) [ 31] at an initial density of 1 × 10 cells/mL.
Because of the labor-intensive effort required in making inoculations, half the plants were inoculated on each of two consecutive days, at the same time each day.
The presence of viable mycoplasmas was determined by inoculating one half of the synovial membrane in l ml of E broth and ten fold serial dilutions were also cultured an inoculated onto agar [ 18].
Within each treatment, we fed half the adult females on a mix of P. berghei ookinetes and blood and inoculated the other half with a negatively charged CM-25 Sephadex bead to evaluate the mosquitoes' melanisation response.
Mr. Bush, who as commander in chief will be inoculated, ordered that half a million members of the armed forces, including those most likely to be involved in any war with Iraq, be immunized immediately.
The third, fourth, and fifth leaves were dusted with sterile Celite® and mechanically inoculated on one half of each leaf.
The non-inoculated half leaves from 25 transgenic plants were also analysed by DAS-ELISA.
