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After a day of shaking to evaporate the solvent, 5 ml of bacterial suspension (≈107) was used to inoculate in 95 ml sterile mineral salt medium.
Agar plugs were cut into small pieces (about 0.5 × 0.5 × 0.5 cm3) under aseptic conditions, 15 pieces were used to inoculate in three Erlenmeyer flasks (250 mL), each containing 50 mL of media (0.4 % glucose, 1 % malt extract, and 0.4 % yeast extract), and the final pH of the media was adjusted to 6.5.
An overnight culture was used to inoculate in CAMHB containing rhodomyrtone at 0.125, 0.25, 0.5, 1, and 2MIC.
Fifty microliters of overnight cultures were used to inoculate in 10 ml of TSB medium pre-incubated anaerobically at 37°C.
Wild-type virus is difficult to control with respect to the amount of virus to inoculate in order to create a reproducible incubation period, clinical picture, and mortality rate.
Effects of the factors "Casts" (±earthworm casts in the microsites), "AMF" (±AMF inoculate in the microsites), and "Earthworms" (±three different earthworm population treatments in the mesocosms) on plant biomass and earthworm performance (fresh weight and cast activity) were analyzed by three-factorial ANOVAs.
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inoculated in the dorsal position in only one inoculation place in each female nu/nu mice.
John Adams was inoculated in 1764.
In the United States, 90.5percentt of children ages 19 months to 35 months had been inoculated in 2000, the latest year for which figures were available.
Then anthers were inoculated in CIM medium without SA.
A. bisporus is inoculated in PII-end compost.
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