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The proportion of the more hydrophilic polymer Eudragit RL had the most significant effect on drug release – higher proportion gave faster release; the amount of inner and outer coat did not have a significant effect on the rate of drug release at either 6 or 12 h in the range studied.
Models for the spatial relationship between inner and outer coat.
How do the inner and outer coat components arrange to form a membrane coat?
White, black and blue arrows point to the membrane, inner, and outer coat layers respectively.
Gaps and dislocations in both inner and outer coat lattices may further contribute to curvature variability.
(D ) A schematic representation of the relationship between inner and outer coat as identifed in this study.
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This arrangement of inner and outer coats results in coated tubular membranes.
A comparison of our results with previous hypotheses for the relationship between inner and outer coats (Stagg et al., 2008; Bhattacharya et al., 2012) is in Figure 6.
We agree with the reviewers that cargo packaging could drive the switch from spherical to tubular morphology, and that the inner and outer coats could co-assemble, with the inner coat imposing regularity on the outer coat, or vice versa.
The data reveal how coat subunits interact with one another and with the membrane, suggesting how coordinated assembly of inner and outer coats can mediate and regulate packaging of vesicles ranging from small spheres to large tubular carriers.
This relationship suggests that the unstructured C-terminal region of Sec31 (Fath et al., 2007; Noble et al., 2012), which connects the inner and outer coats, constrains the coat layers but does not fix their absolute positions, much like multiple anchor cables on a ship.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com