The injured samples can be locally repaired with high precision and efficiency without an obvious influence on those uninjured parts.
A total of 16 injured samples were allocated to histological and immunohistochemical analysis (comprising four in each group) and another 16 allocated to western blotting test (four in each group).
To visualise group differences, geometric mean calculations were performed to express power density values of patients as a percentage of controls, a method previously used in nonbrain injured samples [ 46– 46].
Hence, our tissue collection scheme did not sample myelinated nerve fibers in uninjured samples, yet injured NL and DL samples contained transected nerve bundles located just proximal to the WE.
Although healthy at the time of sample collection, the captive rehabilitated sea turtles were known to have been sick or injured before sampling, potentially reflecting immunocompromise.
Patients are severely injured, and sampling of blood plasma and brain microdialysates at the desirable time points for PK measurements are naturally subordinate to the clinical needs of the patient, which are paramount.
In a self-injuring sample of young adults, 80% met exclusion criterion F [ 53], as did 98.2% of adolescents [ 52].
In a self-injuring sample of inpatient and intensive outpatient adolescents and young adults, 85.5% endorsed criterion A, i.e., at least 5 days [ 52].
Sampling procedures comply with the ARRIVE guidelines; no laboratory experiments were carried out, and no animals were injured during DNA sampling (blood samples taken in tarsal vein; complying with the Swedish Board of Agriculture's ethical standards).
However, the low temporal and spatial resolution limit any detailed information concerning changes between the individual blood sampling time points and influences of injured versus non-injured or lesser injured tissue because blood samples were drawn in four- to six-hour intervals and jugular venous blood reflects global rather than local intracerebral changes.
In summary, the selection problems addressed above may lead to selection bias amongst both adults and children leading to an overestimation of the proportion being injured in this sample altogether.
