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The contents were resuspended in 100 µL of mobile phase (80% methanol and 20% water) and transferred into high performance liquid chromatography (HPLC) injector vials.
Samples were re-dissolved in water (50 µl), centrifuged (16,000× g, 5 min) and the supernatant transferred to LC injector vials.
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The dried residue was reconstituted with 100 µL of mobile phase and transferred to a glass insert inside a 2 mL standard HPLC auto injector vial.
Insulin injectors used vial and syringe only (n = 115), pen only (n = 64) and both vial and syringe and pen (n = 22).
1.5 ml of prepared solution was transferred into a clean injector auto HPLC vials for automatic injection.
The sharing of injecting equipment or drug vials among IPED injectors at 8.9% is much less common than among injectors of psychoactive drugs (in 2011, 37% of psychoactive drug injectors reported recently sharing injecting equipment 40).
33 Switching from a syringe and vial to an injector pen has also been shown to reduce the risk of hypoglycemic events by 50%, 68 as well as to reduce the cost associated with hypoglycemia (see Table 3).
We used Menommune quadrivalent polysaccharide meningococcal vaccine (Aventis Pasteur [formerly Pasteur Merieux Connaught], Swiftwater, PA) in 50-dose vials originally designed for jet injector use.
After exposure for 40 minutes in the headspace, the HSPME fiber was retracted from the vial and desorpted in the injector (250°C) for 2 minutes in splitless mode.
After sampling, the SPME fibre was withdrawn into the needle, removed from the vial and inserted into the injector port (250°C) of the GC-qMS system for 6 min, wherein the metabolites were thermally desorbed and transferred directly to the analytical column.
We also included the effects of block, injector and the number of females in the vial.
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