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This GCMS system equipped with a split/split less injector (set at 10 1) was used to analyse the derivatives of fatty acids.
The analysis was performed using GC MS (Thermo Finnigan trace DSQ II) equipped with a fused silica HP-5MS capillary column (30 m × 0.25 mm ID × 0.25 μm df). 1 μL of sample was injected in a split/splitless injector set at 250 °C.
Figure 3 a c shows the temperature dependence of focusing results with injector set to 0.5G 0, G 0 and 1.8G 0, respectively, where the lattice temperature is incremented from 20 mK (the electron temperature is calibrated to be around 70 mK) to 1.8 K, and Fig. 3 d f shows the zoom-in of the data in Fig. 3 a c, respectively.
The GC-MS analysis was performed on the saponified and esterified component of wax esters; the oven temperature was programmed from 150°C to 200°C at 5°C/min and then at 325°C at ramp 3°C; hold at this temperature for 20 min. The split ratio was 1 10, and helium was used as a carrier gas with the flow rate of 0.8 ml/min, with the injector set at 300°C transfer line set at 325°C.
One μL of each sample was injected into the split/splitless injector set to a split ratio of 1 2.
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The injector setting was a pulsed spit less mode with a temperature of 250°C at a standard pressure.
The split injector was set at 1∶40, and the injector temperature was set at 250°C.
The temperature of sample injector was set to 320 °C.
During desorption of MVOCs from the fibre, the injector was set to the splitless mode.
The splitless injector was set to 280°C with a 30 seconds purge off time.
The injector was set to auto clean itself by pre-injecting hexane.
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