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Used flow velocities ranged between 0,1 µL/s and 100 µL/s, were controlled by the injector pump and were calibrated by measuring flow rates at various pump speeds.
The analysis was performed on a Waters 1525 reversed-phase HPLC system (SpectraLab Scientific Inc, Alexandria, VA) that consisted of a manual injector, pump, and fluorescence detector.
For stereotaxic injection of Lentiviral vectors (LV -eGFP or LV -eGFPsin-eGFP-IRES-Cre (Bateup et al., 2013) intorthe hippocampus of P15 Sema5A (flox/−) mice, an automated injector pump (StoetLV-synapsin-eGFP-IRES-Cre Hamilton syringetwal used.
Figure 6 demonstrates a video of simultaneous structural and Doppler OCT imaging of a porcine carotid artery, where dilute (1.5%) blood in saline flush is injected with a contrast injector pump at 5 ml/s (note: typical human carotid angiography uses 4 to 6 ml/s injection rate of contrast).
Alternatively you could use another fuel tank but remembering that the return pipe from the injector pump or the injectors themselves would have to hooked to it.
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Dropping the throttle sets into motion a Saturn V-style launch sequence: fuel injectors pump, the engine draws a breath, the turbochargers spool and – ignition – the world simply blurs.
Thereafter, an automatic micro-injector pump (kdScientific) with a 10 μl Hamilton Syringe was positioned above the exposed dura.
Afterwards an automatic micro-injector pump (kdScientific) with 10 μl Hamilton Microliter™ Syringe was positioned above the exposed dura.
We describe and compare two protocols commonly used to infect Drosophila with P. aeruginosa: needle-pricking and injector-pumping.
Finally, thoracic injector-pumping delivered in 64±5% CFUs in the thorax (site of inoculation) and 36±5% in the abdomen (remote site).
A new plunger piston matching parts of fuel injection pump and injector nozzle matching parts were designed.
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