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The observed Ca2+ signal begins at the injected cell 30 s into optical injection, spreads sequentially by degree of separation to all cells in the experimental FOV, then returns to near baseline at 120 s (60 s after optical injection turned off).
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Also, it is shown that some improvements are possible when it comes to model the steady-state behavior of the controllable bearing with the injection system turned off.
In the case of 25%% pollutant concentration Table 3, the efficiency of removed oil after first air flushing is 63 wt% and increased sharply tells to 91 wt% at seventh air flushing, this can attributed to air injection is temporarily turned off the water flows into the air channels and mixed with entrapped oil and formed emulsion.
The experimental sequence for initiating and monitoring Ca2+ signaling during optical injection was as follows: 30 s of baseline images (representative of [Ca2+]baseline in each cell), 10, 60, or 120 s of images during optical injection, then 30 or 60 s of recovery images after the optical injection beam had been turned off.
Initially only one response was required to obtain each 10 µg/kg intravenous injection of nicotine (one-response fixed-ratio schedule of reinforcement; FR 1) and after each injection the green light was turned off for 5 sec (5-sec time-out; TO 5 sec).
Immediately after UV lamp was turned off, the injection of precursors of Ag, Au, and Pd caused the rapid nucleation because photoreduced phosphotungstates as well as electrons stored in r-GO directly reduced metal ions.
This bioclogging effect became evident when the injection of the glucose solution was turned off and water flow still bypassed the area around the glucose injection port, preserving the flow pattern as it was during the injection of the glucose solution.
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