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Doubled injection time is employed to inject the same amount of fluids.
The amount of the injected sample was controlled by the pressure and the injection time.
Antibodies were injected in triplicate at 100 nM at a flow rate of 30 μl/minute, injection time two minutes, post-injection time ten minutes, followed by regeneration of chips with 5 μl 0.1% tri-fluro acetic acid (TFA).
140 min before 8-OH-DPAT injection (time 0 min).
A maximum injection time of 60 ms was used for ion accumulation.
HCD collision energy was set to 27, AGC target to 5e4 and maximum injection time to 200 msec.
The resolution was set to 17,500 at 200 m/z combined with a maximum injection time of 60 ms.
The two parameters that are predicted from the developed network are injection time and injection pressure.
The plate is designed for injection time, maximum mold pressure, stiffness and buckling.
Blood samples were collected from −10 to 150 min relative to injection time.
The wellhead pressure and bottomhole temperature behavior with injection time are predicted.
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