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Conditions: carrier gas hydrogen (1.5 mL/min); sample diluted with MeOH (1 1000); splitless; injection temp.
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Separation method: 1 μL injection; injector temp: 250 °C; detector temp: 220 °C.
300 °C; carrier gas He, flow rate 1.0 ml/min, constant pressure mode; splitless injection, column temp.
Set = intersection(Set, Temp).
Following the injection with DLW, bats were held at room temp for 1 h for isotopic equilibration, after which time they were anesthetized with 5% isoflurane and decapitated.
The separation was performed using the following parameters (capillary temp: 60°C, denature temp: 90°C, time 150 s, injection voltage: 2 kV, time 7 s, separation voltage 3 kV and separation time 100 min).
Typical run module parameters were: Run temp = 60 °C, Capillary fill volume =184 steps, Pre run voltage =15 kV, Pre run time = 60 sec, Injection Voltage = 1kV, Injection time = 13 sec, Run Voltage = 15 kV, Data Delay = 200 sec, and Run time = 1200.
The temp. was 92.
Maximum temp: 22C.
Min temp: 15C.
Max temp: 21C.
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