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The hyperpolarized 13C solution was extracted from the vial and injected into a custom-made injection pump equipped with a 13C NMR probe (Alderman-Grant coil tuned to 100.67 MHz)27,37.
As contrast agent, we used 0.1 mmol /kg body weight of gadolinium-diethylene-triamine-pentaacetic acid (Gd-DTPA) (Magnevist, Schering, Berlin, Germany), which was injected by a pneumatically driven injection pump at an injection rate of 5 mL /s.
A single dose of pilocarpine hydrochloride (1.2 mg/ μL, total volume 2 μL; Sigma-Aldrich, USA) was injected through a needle connected to an injection pump (flow rate: 1 μL/min; Stoelting Co., IL, USA).
A 50-ml intravenous bolus of a low-osmolar non-ionic contrast agent with an iodine concentration of 300 mg/ml (Ultravist-300 Iopromide; Schering A.G., Berlin, Germany) was injected over 6.25 s (8 ml/s), using an injection pump through an 18-gauge needle.
It had been modified in 1943 with the addition of a Bosch fuel injection pump.
Firstly, the paper total analyses the injection pump durability test.
In Supplementary Note 3 we fit the distribution of chemical injection pump measurements to several functions characteristic of skewed distributions.
This injection pump is designed for rodent experiments and equipped with a 13C NMR probe (see ref. 27 for details).
(f) Liquid-state 13C NMR longitudinal relaxation measurement performed at 9.4 T and room temperature inside the injection pump.
Parts like the diaphragm in the fuel injection pump "dry out and crack".
In general this process is highly dependent upon the injection pump and fuel injector health.
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