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One injection of the compound given on the day of birth seemed to work by allowing the animals' cerebellums to grow to full size.
The injection of the compound EPSCs was made from an average resting potential of −65±0.37 mV (mean±s.e.m ., the mean voltage depolarization averaged over all suprathreshold compound EPSCs kinetics was 25.2±0.79 mV (mean±s.e.m).m
A similar suppression of growth was achieved with peitoneal tumours by the intraperitoneal injection of the compound.
Fifty minutes post injection of the compound, a 15 minute scan will be acquired using a Phillips Allegro PET camera.
Because of the natural disease course of insulinoma in fasting patients, blood pressure and glucose values were monitored before and after injection of the compound at several time points.
T/nT and K/nT ratios were assessed for SPECT scans performed between 2 and 3 h, 3 and 4 h, 5 and 6 h and 24 h (in two patients) after the injection of the compound.
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Tumor-bearing mice, as verified by bioluminescence, were treated by i.p. injection of the compounds starting on day four post-implantation.
Glucose was measured before injection of the compounds (t = 0) and after 15, 30, 45, 60, 90 and 180 min. Blood was drawn either by retro-orbital bleeding or by cardiac puncture for triglyceride, free fatty acid and ketone body measurements.
Mice were sacrificed 16 days after the first injection of the compounds and blood sera were collected.
Rats received daily injections of the compound during their first 4 days of life, a developmental period that corresponds to the second to early third trimester in human gestation.
At 30 minutes after contusion injury, mice were treated with arachidonyl trifluoromethyl ketone (AACOCF3; Cayman Chemicals, Ann Arbor, MI), delivered intravenously (50μl of 4mM), followed by intraperitoneal injections of the compound (200μl of 4mM) every other day up to 2 weeks postinjury.
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