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Following virus injection, animals were intravenously injected with ∼250 µCi of [18F]FEAU tracer 2 hours before PET imaging was performed.
After injection, animals were inspected every 2 days and the tumour's length and width were measured by a caliper.
Three weeks after injection, animals were euthanized and explanted specimens were analyzed by microcomputerized tomography (micro-CT) and histology.
At 1 h, 6 h, and 7 days after injection, animals were anesthetized with sodium pentobarbital and killed via cardiac puncture.
At 30 min post injection, animals were sacrificed under isoflurane anaesthesia (2% in O2 at 1 L · min−1) by cervical dislocation.
Seven days after injection, animals were perfused as indicated below.
Following icv injection, animals were sacrificed by decapitation, and the brain was removed.
After injection, animals were normally maintained and fed, and were observed for 7 days.
After 24 hours of the last injection, animals were sacrificed for various assays.
Nine weeks after starting injection, animals were euthanized by CO2 narcosis.
After recovery from anesthesia and 45 minutes after injection, animals underwent systemic kainate administration.
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