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Fast Blue injection and tissue processing were as described above.
The animals were sacrificed 2 h post injection and tissue samples were collected and fixed either in 70% ethanol or 10% NBF.
As shown in Figure 4A, poly(I∶C) rapidly induced villous atrophy within 6 hours of injection and tissue damage was maintained through the 12 hour time-point as reported previously [26].
LNK participated in viral injection and tissue processing.
SKK and LVK participated in the design of the study, viral injection and tissue processing.
Muscle protein synthesis was calculated as FSR (%/h) = E(protein-bound)/(E(muscle free) × t) × 100, where t represents the time interval between phenylalanine injection and tissue sampling.
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Mice were sacrificed 14 days after injection and tissues were isolated and fixed in 10% neutral-buffered formalin.
Mice were euthanized 14 days post injection and tissues such as spleen and tumour were removed for experimentation.
Oestrus was synchronized in Holstein cows by prostaglandin injection and tissues were collected during the luteal and peri-oestrous stages of the oestrous cycle.
Mice were killed 14 days after injection and tissues were isolated and fixed in 10% neutral-buffered formalin.
Animals were then euthanized at 4 h post injection and tissues explanted, weighed and gamma counted on a gamma counter (Wallac, 1282 Compugamma, PerkinElmer, UK).
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