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APN wild-type or site-directed mutant cRNA (15 ng per oocyte) was injected into the oocyte, the injection amount of all other cRNAs having been previously optimized [ 11, 17].
The new isoform- and vector-injected oocytes were paired into groups based on injection amount.
The fuel injection amount and timing will be adjusted according to the fuel estimation result and the feedback control algorithms.
Subsequently, in a century-long simulation, a feedback algorithm was employed to meet specific objectives and manage uncertainty, wherein the injection amount at each location was adjusted every model year.
Moreover, some principal parameters, which include the permeability, anisotropy, total injection amount, and length of the injection part of wellbores, were investigated by numerical simulations.
The paper finally investigated the effect of crude oil price on the optimum injection amount of polymer solution for a given reservoir.
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Cycle increment value of injected steam is 10%%, and the injection amounts of dissolver and carbon dioxide increase correspondingly.
One dramatic example came in 2008, in Baze v. Rees, which asked whether execution by lethal injection amounted to cruel and unusual punishment, in violation of the Eighth Amendment.
Injection amounts varied from 0 to 15 times the amount of GABAB1a injection (Figure 6).
The vector controls also had the same injection amounts, but their GABA responses were constant and stable.
Except for the GABAB1l and m injection ranges that decreased GIRK currents, other injection amounts did not show the inhibitory effects.
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