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The cylinder was then disconnected and a mixture of ethanol and distilled-deionized water (1 1) was passed through the columns at a flow-rate of 1 mL/min for a further 10 min. The columns were then equilibrated with of 0.04 M phosphate buffer (0.04 M, pH 3.4) at a flow-rate of 1 mL/min for 30 min. A 400 μL aliquot of FA sample was loaded into the injection valve and then injected into MI.
The experiments simulated a pipe rupture in a cooling plate of the HCLL TBM, injecting He from an injection valve connected to He-FUS3 loop.
A sample line (5) connected to the sample reservoir (not shown) carries the sample fluid to the injection valve (6) where it is injected into the flow of the inlet buffer.
The samples were injected onto a column using an injection valve, a model 7225i from Rheodyne (Cotati, CA, USA).
The samples were injected onto a column using an injection valve, Rheodyne Model 7225i (Cotati, CA, USA).
After hydrodynamic equilibrium was established, as indicated by a clear mobile phase eluting at the tail outlet, the sample solution (4.0 g in 50 mL of each phase) was injected into the separation column through the injection valve.
A Gilson 234 autoinjector (Villiers-le-Bel, France) equipped with a Rheodyne (Bensheim, Germany) six-port injection valve (different injection loops) was used to inject samples.
It consists of 3 independent inlets for 3 different mobile phases with an in-built degasser, 4 peek solenoid valves, 2 independent nano piston pumps, 2 selectors (6/7), 2 manual inject valves with 100 μl loop volume (adjustable), 2/10 valve, 3 check valves and unique automated manifold for inserting microfluidic chip.
The computer-controlled pneumatic microvalve enables volumes ranging from picoliters to nanoliters to be injected based on valve opening time and injection pressure, and a seamless interface from microchannel to capillary prevents sample losses and large dead volumes.
Samples were injected into the flow of DIW by a manual six-port injection valve (Rheodyne, USA) with a 0.6 mL sample loop volume unless stated otherwise.
The precursors are injected through pneumatic valves in a heated valve box.
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