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The 100 µL of ProCA1-affi-m was injected to each xenograft by i.v. injection.
Subsequently, 1-mL E. coli suspension of 107 CFU/mL was injected to each sample.
Approximately 800 ng of chitin was intraperitoneally injected to each mouse and peritoneal exudate cells (PECs) were collected 1 d after chitin injection for flow cytometry and gene expression analysis.
Two ml of 0.5% bupivacaine was injected to each site, which were approximately 2 cm lateral and 1 cm inferior to the external occipital protuberance, just medial to the occipital artery.
A total of 50 nL were injected to each oocyte, delivering a mRNA mass total of 25 ng.
Basal fluorescence intensity was measured for three time, and then 10 μl of cocktail of 10 ng activated JNK (JNK a))or inactive JNK (JNK(m)) and 600 μM ATP with or without 10 μM FeTCPP was injected to each well.
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Resting membrane potentials were measured immediately after electrodes broke into the cells following formation of a gigaohm seal, and current pulses were injected to examine each cell's basic electrophysiological properties.
Then, a location method based on this energy is proposed in [49] by monitoring the energy injected to the network at each port and identifying the port with positive energy injection as the source.
As explained further in "Learning a control policy" to allow for more efficient execution of the learning and simulation process, a single signal (state value) is injected to all control nodes at each time step.
Double the volume of saline was injected to replace blood lost after each sampling.
1 μL of each sample was injected to the GC-ECD for separation and quantitative analysis.
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