Exact(3)
An algorithm that can identify the initiation sequence for the hole-by-hole blast is developed from the Voronoi diagram.
cDNA encoding scFv-6E (GenBank accession number FJ695518) was amplified by PCR using complementary primers that introduced a 5' Kozak initiation sequence for mammalian expression and a 3' HA epitope (YPYDVPDYA) for biochemical detection.
All PCR primers contained a 5′ optimized Kozak initiation sequence for efficient translation in yeast [ 35] and either a 5′ XhoI or EcoRV site and a 3′ NotI site (primer sequences are shown in Additional file 1: Table S5).
Similar(57)
Using BIMAS ProScan software [ 12], we identified putative transcription initiation sequences (for example, TATA or initiator elements) within the proximal sequences, which can be indicative of promoters.
Unfortunately, we do not know if a shorter initiation sequence is sufficient or if a longer sequence is required for optimal results.
Sense primers used for PTT contain a T7 promoter and a eukaryotic translation initiation sequence: 5' -GGATCCTAATACGACTCACTATAGGGA-GACCACCATG-3'.
A method is proposed for bench blasting in open pit mines that can automatically determine the blastholes positions, calculate the explosive charge mass, and identifies the initiation sequence in the case of the hole-by-hole initiation.
Moreover, detailed insight into promoter and translation initiation sequences should be valuable for the future development of new and better expression tools for B. methanolicus.
(B ) Comparison of frequencies of various translation initiation sequences (−4 to +3) for annotated ORFs (x axis) and AUG present in 5' leaders (y axis).
Executive functioning represents the cognitive skills required for the planning, initiation, sequencing, and monitoring of complex goal-directed behavior, such as household chores [ 5, 62, 63].
The Origin Recognition Complex (ORC) nucleates preRC assembly on DNA [1], thus identifying the target sequence for initiation.
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