All animals were killed for histologic examination 8 weeks after the initiation of the experiment.
One cow was excluded before initiation of the experiment because of complications from mastitis.
Samples were taken 0, 2, 5, 10, 20, 30 and 60 min after initiation of the experiment.
Similarly, cows consuming Bio-Mos were better able to maintain BCS from initiation of the experiment through weaning (P = 0.05).
Microbial activity in the added synthetic porewater in the borehole was apparent shortly after initiation of the experiment and affected the geochemical parameters observed in the porewater.
Concentrations of CCK were determined in jugular plasma obtained from each lamb (4 h after feeding) 6 weeks after the initiation of the experiment.
The animals were allowed 7 days to acclimate and were given ad libitum access to standard rat chow (0.5% NaCl) (Baxter Healthcare Corporation, Deerfield, IL, USA) and tap water until the initiation of the experiment [14].
The ELISA was able to detect quantitatively the significant acquired primary and secondary immunity to the vaccine in T3 and T4 ewes, compared to their low level of background immunities at initiation of the experiment (p < 0.05).
Changes in root exudation profiles induced by our treatments became most marked 14 and 21 days after initiation of the experiment; on those days, we observed (i) increased fatty acid exudation from seedlings treated only with humic acids and (ii) increased exudations of nitrogenated compounds and terpenes from seedlings treated only with H. seropedicae.
Additionally, the effect of the pressure build-up time (specific to the experimental set-up at hand) on the processing targets was quantified based on the microbial and enzymatic activity load before the initiation of the experiment and after the stabilisation of the treatment conditions.
Laboratory tests were performed before initiation of the experiment and at week seven.
