Finally, EvoPrinterHD incorporates options that allow for (1) re-initiation of the analysis using a different genome's aligning region as the reference DNA to detect species-specific changes in less-conserved regions, (2) rapid extraction and curation of conserved sequences, and (3) for bacteria, identifies unique or uniquely shared sequences present in subsets of genomes.
Due to non-proportionality of period of initiation the analysis was stratified for period of initiation of HAART.
If several alternative transcription start sites (TSS) had been identified for a transcript, we used the TSS furthest from the site of translation initiation in the analysis, to minimize the loss of sequence information.
TT initiation of the study, design, analysis and co-writing.
RM: initiation of the study, design, analysis and writing.
Multiple testing is another issue to be considered in this study, as several analyses were conducted and we combined various risk factors; however, this concern should be mitigated by the fact that the hypotheses were predefined and the exploratory evaluation of the combination of variables was planned before the initiation of any analysis.
A Statistical Analysis Plan (SAP) has been developed prior to initiation of the registry and final analysis of the trial will be performed by syneed medidata GmbH.
DM: initiation of the study, design, implementation, analysis and writing.
FA: conception and initiation of the study, design, implementation, analysis and drafting the manuscript.
