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The fire and grazing treatments were in place prior to the initiation of sampling in 2011, so all patches and pastures had a consistent elapsed time since fire (0.3, 1, 2 years since fire) (Table 1).
All cultures were shaken approximately 1.5 hrs prior to initiation of sampling (18).
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It is beneficial and is recommended to have a well-defined plan for the characterization of ADA domain specificity and data analysis prior to the initiation of sample testing.
All owners signed an informed consent form before the initiation of sample collection.
Time between CTP addition and initiation of sample recording averaged 5 s.
It is therefore important that issues surrounding biopiracy are addressed before the initiation of sample and data collection.
Time between glutamine addition and initiation of sample recording averaged 5 s was based on the amount of time required to load the sample.
A distinctly different pattern was observed in one serial CSF sampling study characterized by a sharp increase in Aβ40 and Aβ42 concentrations immediately after initiation of sample collection and followed by a rapidly achieved plateau that was sustained through the remaining sample collection (Figs. 1e and 2e).
At the initiation of blood sampling some subjects may present stress-induced elevations in the plasma hormone concentrations due to unfamiliarity with the clinical procedure.
Duration of ART was calculated from the day of initiation of ART until the time of sampling.
The study set-up included 4 time points for each patient where both plasma samples had been frozen and quarterly blood analysis had been performed: Sample 1: last sample before initiation of ART, Sample 2: three months after initiation of ART, Sample 3: one year before case's MI/index date, and Sample 4: last sample before case's MI/index date.
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