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Initiation of assembly involves FN binding to cell-surface receptors.
This Ca2+-regulated switch between structural states allows for the purification of monomeric proteins followed by the removal of Ca2+ and the initiation of assembly.
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These include its initial recruitment, the initiation of oligomer assembly, GTP hydrolysis, and membrane constriction, scission, and finally, oligomer disassembly 73.
This was proposed to 'kickstart' initiation of pseudopilus assembly, possibly by activating the assembly ATPase in the cytoplasm (although this remains purely speculative).
High-resolution SEM (HR-SEM) analysis of the 5 mg/mL samples taken minutes or hours after the initiation of the assembly process indicated that large aggregates are present at the initial stage when the solution is turbid, whereas after several hours, ordered structures with much smaller diameter appear, corresponding to a considerably clearer solution.
In this regard, initiation of heterochromatin assembly by the ClrC complex has been proposed as an alternative model.
Here we propose that Cdc20, the DNA Pol ε subunit, as a central player driving the initiation of heterochromatin assembly.
Deletion of cofB produces a phenotype with no detectable pili formation on the cell surface, while molecular modeling indicates that the characteristic homo-trimeric structure of CofB is well situated at the pilus tip of colonization factor antigen/III formed by the major pilin CofA, suggesting a role for the minor pilin in the efficient initiation of T4P assembly.
This initiation of tubulin assembly is called microtubule nucleation [3], [4].
The relocation of ZO-1 to cell cell junctions represents an important step in the initiation of TJ assembly [9].
To probe the effect of a compound on MT stability the molecule was added 5 min after initiation of tubulin assembly.
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