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For example, the actinorhodin and tetracenomycin minimal PKSs could accept and elongate unnatural primer units from the R1128 initiation module.
Nine subdomains encoding diverse specificities were transplanted into the Phe-specific GrsA initiation module of gramicidin S synthetase.
These polyketides are synthesized by bimodular PKSs comprising of a dedicated initiation module, which includes a ketosynthase, acyl transferase and ACP, as well as a minimal PKS module.
Therefore, the first module of XALc_1057 was assigned as the initiation module of META-B.
Three NRPS gene clusters (Cluster 1 3) were identified from four Subsection V cyanobacterial genomes which lack a C domain in the initiation module.
It is unclear if these gene clusters are incomplete (missing an initiation module) or if these gene clusters encode an NRPS natural product.
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Recently we showed that regioselectively modified polyketides could be synthesized through the genetic recombination of initiation modules and minimal PKS modules from different polyketide biosynthetic pathways (Tang et al. PLoS Biol. 2004, 2, 227 238).
Typically, NRPS initiation modules lack a C-domain [ 3].
The exceptions are initiation modules of NRPS involved in biosynthesis of cyclic lipopeptides [ 5].
This high proportion of AS domains within initiation modules within the Subsection V cyanobacteria is therefore unique.
AS domains have only been identified as initiation modules in these three NRPS/PKS gene clusters within cyanobacteria.
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