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Nogales, E., Louder, R. K. & He, Y. Structural Insights into the eukaryotic transcription initiation machinery.
Kuzmenko, A. et al. Mitochondrial translation initiation machinery: conservation and diversification.
A key step of translation is recruitment of initiation machinery to the 5′ mRNA cap.
In addition, METTL3 can recruit EIF3 to interact with translation initiation machinery and promoted translation of m6A-modified mRNAs independent of its methyltransferase activity23.
Eukaryotic translation initiation factor 4E (eis4E) is considered as the corner stone in the cap-dependent translation initiation machinery.
Yeast eIF3 engages 40S in a clamp-like manner, fully encircling 40S to position key initiation factors on opposite ends of the mRNA channel, providing a platform for the recruitment, assembly, and regulation of the translation initiation machinery.
As our polysome-profiling results suggested a role of Xrn1 in translation initiation (Fig. 2b), we examined whether Xrn1 interacts with the eIF4F complex, a key component of the translation initiation machinery that binds to capped mRNA and mediates its interaction with the 43S pre-initiation complex20.
This results in defective PTEN function and unrestrained Akt signaling leading to downstream activation of the translation initiation machinery [16].
Second, Rbf1 may inhibit activity of the replication initiation machinery after it has assembled at an origin of replication.
The interaction with 4EHP-Prep1 would sequester the target mRNA preventing its association with the translation initiation machinery.
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Ssu72 also functionally interacts with other components of the transcription pre-initiation machinery (e.g., TFIIB) (Pappas and Hampsey, 2000) and may facilitate interactions between the 5′ and 3′ ends of genes, promoting gene "loops" (Tan-Wong et al., 2012).
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