In the preparation course of a BRI/PHEMA contact lens, the monomer HEMA and the PEG-DMA were co-polymerized by initiating with a UV light free radical initiator D2959.
Nevertheless, CKD pathogenesis shares the characteristic of escalating fibrosis, often initiating with a primary injury (cell loss or abnormal behavior) located at the glomerulus or tubule (Figure 4C).
Further analysis showed that this strong enrichment of miRNA* sequences primarily concerns those initiating with a 5'G resulting in miRNA* sequences with a 5'G as the predominant miRNA* species in infected cells (Figure 4A).
It should be noted, however, that size- and 5'-terminal guanine are unlikely to represent sufficient criteria to dictate sRNA enrichment since some 21-nt siRNAs initiating with a 5'G are not enriched upon ORMV infection (data not shown).
Whereas the number of sequenced unique miRNA* sequences initiating with a 5'G is increased from 13 to 15 upon infection, their total number is increased 45 fold, i.e. from less than 500 reads to more than 20000 reads (Table S5).
The increase in accumulation seen for sRNAs initiating with a 5'U is to the majority caused by virus-enriched miRNAs, whereas the increase seen for sRNAs starting with a 5'G reflects the accumulation of miRNA* sequences (see also Figure 4A).
