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He became adept at initiating experiments that played to the current Moscow fashion, and then pushing them to the brink of heresy.
Cells were cultured in YEPD (1% yeast extract, 2% peptone, 2% glucose) and maintained in exponential growth for 15 hr to a maximum density of 5 × 10 cells ml−1 before initiating experiments.
Prior to initiating experiments, cells were transferred to NDiff N2B27 (StemCells Inc, SCS-SF-NB-02) supplemented with 100 U/mL LIF and 10 ng/mL BMP4 (Department of Biochemistry, University of Cambridge) or 3 µM Chiron (CHIR99021, Division of signal transduction, University of Dundee) and 2 µM PD03 (PDivision, Divisiof of signal transduction, University of Dundee) and cultured for two passages.
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The values at 343 K for both thermally and photochemically initiated experiments were found to be consistent, suggesting that photoinitiated systems may polymerise in the ground state.
We designed the MU in a way that it not only takes care about configuration and collecting violations, but also initiates experiments, monitors all CAN buses individually, stores the test results and performs a preliminary analysis.
To investigate if miRNA activity is sufficient for regulation of early osteogenic differentiation, we initiated experiments with miRNA mimics.
In order to compare single-cell RT-PCR analysis to flow cytometry analysis for IAV proteins, we initiated experiments using the DC-like cell line XS106.
Toward the ultimate goal of understanding how arbuscules develop, we initiated experiments to identify genes expressed in cortical cells containing arbuscules.
Therefore, we initiated experiments to investigate the neutralization activities to heterosubtypic influenza viruses in serum samples collected from the recipients of the 2009 pandemic H1N1 influenza vaccine.
We have initiated experiments to investigate the mechanisms responsible for the enhancement of antitumour activity by HGS-ETR1 in combination with chemotherapeutic agents.
When adherent cells became ~80% confluent (approximately day 4-6 of culture), nonadherent cells were washed away and cells were passaged onto 3- to 150-mm culture dishes and then immediately used to initiate experiments or were cryopreserved.
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