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These data provide further evidence that LARGE possesses poor initiating activity but works efficiently in polymer formation once B4GAT1 is added for initial transfer of GlcA.
We have identified a monovalent deoxynojirimycin (DNJ) derivative that inhibits the CaBmt1-catalyzed initiating activity, and mono-, tetra- and polyvalent deoxymannojirimycin (DMJ) that modulate the CaBmt1 activity toward the formation of a single major product.
It is quite another matter to argue successfully that the initiating activity has the particular mentalistic attributes that volitionalism has characteristically ascribed to acts of willing.
Interestingly, a prolonged engraftment was observed upon transplantation of ROShigh cells in contrast to faster leukemia repopulation in recipients transplanted with ROSlow cells, showing that the metabolic activity is indicative for its leukemia initiating activity.
In addition to clonogenic activity, we also compared tumor initiating activity of CD133-positive and ALDHhigh cells.
Suva et al recently showed that CD133 expression marks a population of Ewing's sarcoma cells with tumor initiating activity [30].
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Indeed, divergent and high frequencies of tumourigenic cells with enriched cancer-initiating activity have been suggested for certain malignancies (Quintana et al, 2008).
Consistent with our findings in the mouse, siRNA studies have demonstrated that OCT4 and NANOG expression are required for human breast tumor-initiating activity [ 62].
None of the HAN initiated gamma-glutamyltranspeptidase (GGT) foci when assayed for tumor-initiating activity in rat liver foci bioassay.
The tumorsphere assays revealed that NOTCH1 is required both for the initiation and maintenance of tumorspheres in vitro and potentially for mammary tumor-initiating activity in vivo.
Gibbs, K.D. Jr. et al. Decoupling of tumor-initiating activity from stable immunophenotype in HoxA9-Meis1-driven AML.
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