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Transcription initiates at a tetracycline-inducible promoter, proceeds through the first reporter (FLUC), and then must traverse the test termination sequence before reaching the second reporter (hRLUC) (Figure 1A).
Artificial termination of read-through transcription from the E1a promoter by insertion of ectopic transcription termination sequences (GGT: globin gene termination sequence) dramatically reduces early E1b gene expression in cis [20] [22].
Immediately downstream of the HAV insertion there was a BGH polyadenylation termination sequence (BGH).
This poly-T tail functions as a termination sequence for RNA polymerase.
These results highlight the versatility of the β-globin element as an effective transcription termination sequence.
During intrinsic termination (also called Rho-independent termination), a stem-loop hairpin encoded in the termination sequence causes the EC to dissociate.
The translation termination sequence of the latter two vectors matched those from vector pCMV2.2-hIgG1Fc-XP.
Each gene in these vectors contain transcription termination sequence of nopaline synthase gene (nos 3′).
All 44 independent plants showed parthenocarpic fruit development, irrespective of the termination sequence used.
This made use of the established ability of such termination sequences to reduce transcription initiated from promoters other than the target.
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