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The freeze-dried samples and reference materials were analysed in triplicate (3 mg) by heating at 50 °C for 45 min initially to remove residual moisture left in the sample and this was followed by heating to 105 °C in alumina crucibles at a heating rate of 20 °C min−1 in nitrogen (BOC, UK) at a flow rate of 50 mL min−1.
Ross was the leader of a $1.1 billion buyout of a troubled Cyprus bank in 2014 that seemed initially to remove Russian owners from the bank's list of equity owners.
A cutoff of 20 reads covering the SNP site was used initially to remove SNP sites with low coverage.
Purification of the UbcH7∼Ub conjugate was performed with a HisTrap column (GE Healthcare) initially to remove UbcH7, and following TEV cleavage to remove His-Uba1.
It was concentrated initially to remove the solvent and then concentrated under vacuum to remove water at temperature less than 60°C.
Daily hemodialysis or continuous hemofiltration may be required initially to remove urea and potassium that are released from damaged muscles [ 142].
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Although they were initially designed to remove endotoxin and cytokines respectively, they might eliminate unexpected mediators responsible for sepsis.
Initially designed to remove proinflammatory cytokines in patients with severe sepsis syndrome, clinicians are now using these membranes for the treatment of myeloma kidney and rhabdomyolysis.
This was evident in both the Facebook page and the response to it by Facebook's management, who, despite the site's anti-bullying policy, initially refused to remove it on grounds that it did not target named individuals.
The earliest biocleaning attempts date back to 1970, initially performed to remove animal glue layers from paper, canvas and polychrome artefacts, and later to hydrolyze glue paste or protein/oily binder [5,6].
The films were initially heated to remove the solvent in the vacuum oven and the temperature was controlled as follows: at 60°C for 4 h, at 80°C for 2 h, then raised to 120°C for 1 h; finally, the films were heated at 160°C for 1 h.
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