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As an alternative procedure for demonstration of SP and NK-1R immunoreactions, sections were initially pretreated with acid potassium permanganate for 2 min, a procedure found to enhance specific immunofluorescence reactions [ 30].
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Cells were initially pretreated for 24 hr with inhibitors, which were then maintained in the culture medium as EMT was induced with 4-OHT for 48 hr.
Each sample was initially pretreated through heating in 20 ml/min of nitrogen from ambient to 300 °C, with a 1 h hold.
The PC12 cells were pretreated with proteasome inhibitor MG132 (30 µM) for 3 hours to initially inhibit the proteosomal degradation of Nrf2, leading to the accumulation of Nrf2 for degradation studies.
Initially, the ability of human breast cancer cells to adhere to bone matrices that had been pretreated with bisphosphonates was investigated by van der Pluijm et al. [ 16].
Pretreated with a cleaning solution, they clean and disinfect with only one product.
Filters were pretreated with 0.1% BSA in Mg-HEPES.
e, Normalized social preference in mice pretreated with i.p.
All samples were pretreated with puromycin before RNA extraction.
Cows were pretreated with progesterone for 3 d.
To test this possibility, neutrophils were pretreated with TLR4 inhibitor before stimulated with OO extract.
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