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Initially, methods employing isolation of intact chloroplasts, and extraction and fractionation on density gradients [42] were explored to obtain chloroplast division machineries.
Initially, methods to estimate the thermal load of buildings are given, including the heat balance method, the transfer function method, heat extraction rate and room temperature, the simple degree-day method, and the building heat transfer.
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Initially, these methods were highly controversial, but, as they were shown to be successful, they became the standard of treatment.
We initially developed methods for efficient genome editing of primary B cells isolated from peripheral blood.
Initially, the methods and models are validated against a series of pigments molecules for which experimental data exist.
Initially, conventional methods were tested for breaching the sand wall.
One of the initially popular methods is double immunodiffusion (Crowle, 1961).
A data safety monitoring board initially reviewed methods and reviewed outcomes every six months thereafter.
Initially, these methods relied on uncharacterized genomic differences or empirically selected target genes.
Initially, statistical methods were exploited with the aim of discovering patterns in positive sequences.
Initially, the methods are described for each step performed in the development process.
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