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Nasal continuous positive airway pressure was employed initially for 1 hour during the first night at home.
London Live, which is due to launch in spring next year, will initially air for 18 hours a day.
London Live, which will launch next year on Freeview, will initially air for 18 hours a day but will become a 24-hour channel if it can attract enough viewers, according to the bid document submitted to Ofcom.
To test strains for sensitivity to metabolic inhibitors, strains were initially grown for 16 hours in YPD medium.
The patients were initially ventilated for 24 hours according to the ARDSNet protocol.
Cells were initially incubated for 3 hours in glucose-free Krebs-Ringer bicarbonate buffer (KRB).
For androgen induction experiments, LNCaP cells were initially grown for 24 hours in standard media and then maintained in charcoal stripped serum supplemented media for 24 hours.
For RBRs that initially rolled for 4 hours and then intermittently afterward (1 hour per 1 or 2 days), some variability is observed in the conversion profile, especially at early reaction times.
The blots were initially blocked for 3 hours with 5% bovine serum albumin in 50 mmol/l Tris-HCl 0.15 M NaCl (pH 7.2; TBS) and then rabbit affinity purified anti-carboxyl-terminal antibodies (0.3 1 μg/ml) and anti-keratocan hybridoma conditioned media (KER-1; 1/100 dilution) were added overnight in 2% bovine serum albumin in TBS.
The neural retinas were initially fixed in 4% paraformaldehyde for 2 hours at 4°C and rinsed in PBS 3 times (5 minutes each).
Chill for 2 hours.
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