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We initially established that the changes in the kinetic and binding parameters were not in consequence of the conformational changes induced in the mutant proteins by ensuring similarity of the mutant proteins to the WT by CD and fluorescence measurements as well as by the susceptibility to trypsin cleavage, all of which indicate a common overall three-dimensional structure.
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Menzies skilfully takes us into his confidence, initially establishing that he is just like us, as he grapples with Shawn's fierce but sometimes fevered text.
Initially, we established that inhibition of MAPKs led to the down-regulation of MMP-2 and CXCR4.
Initially, we established that knockdown of myospheroid led to a significant decrease in cell speed (mean cell speed 1.54±0.11 µm.min−1) when compared to wild-type (mean cell speed 2.77±0.60 µm.min−1) in a similar manner as observed in the mys hemocytes (Fig. 1E).
Indeed, so successful were the results from the first TOH that, in 1904, Cassel provided the funds for a second one, initially established at Fayoum.
Borrowing some ideas (and the name) from a successful Boston program, Slutkin initially established an approach that exists in one form or another in many cities: outreach workers tried to get youth and young adults into school or to help them find jobs.
Culture and partnership: A partnership with an emergency medicine national leader was initially established via relationships that RS already had in place.
The phytotoxic activity of -catechin, a flavonoid exuded from roots of Centaurea maculosa, was initially established in experiments that used Murashige and Skoog (MS) basal medium [5], [6].
Adult green sharpshooter (GSS) Draeculacephala minerva were obtained from laboratory cultures (Rodrigo Almeida, UC Berkeley) initially established from insects that were wild-caught in and around Berkeley, CA. Digital PCR was used to determine the genome copy numbers of individual Sulcia cells.
Finally, complete disclosure is achieved by presenting the technical specifications of the BAFSA, which fulfills all the requirements that were initially established as design criteria.
Sometime prior to 1.5 billion years ago a phagotrophic eukaryote engulfed a cyanobacterium that was initially established as a permanent endosymbiont [ 1, 2].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com