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The mapped GO terms were initially annotated with the default annotation threshold (55) and un-annotated sequences were further annotated at a threshold of 45.
The list of statistically significant transcripts was initially annotated using the Affymetrix NetAffyx annotation file to match a representative Genbank public ID and Unigene to each probeset.
2 Hits that involved a majority of nucleotides that were in between annotated ORFs. 3 ORFs that were not initially annotated as transposases of the PSSM IS family but were hits of the PSI-BLAST search.
Two mRNA sequences (AB050197 and BC019095) were initially annotated upstream and downstream of PCA3.
In fact, the esterase was a cell wall-associated carboxyl esterase rather than a protease as initially annotated.
The ORF S009 initially annotated in the SGI1 sequence revealed to be non-functional, thus the insertion of the kanamycin marker gene would not affect the transfer of SGI1 (B. Doublet, unpublished results).
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Users can (i) initially annotate CB regions in input protein or nucleotide sequences of interest, and then (ii) query a database of greater than 1 500 000 pre-calculated protein-CB regions, for investigation of further functional hypotheses and inferences, about the specific CB regions that were discovered, and their protein disorder propensities.
Initially, we annotated the multisample VCF with the SnpEff genetic variant annotation and effect prediction toolbox [ 12].
Initially, 70 annotated genes of P. carinii were retrieved from Genbank.
New paralogous sequences, initially not annotated, were found thanks to this method and integrated in the final batch of proteins.
Initially, we annotated the human and mouse genomes with STRs (see Figure 1 for an example and Methods for how STRs are identified).
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