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Monkeys were initially anaesthetized as above.
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The rats were anaesthetized as above.
Rats in both experimental groups were initially anaesthetized using isoflurane (Abbot Laboratories, Maidenhead, UK) in an induction box.
The animals were initially anaesthetized with 60 mg/kg pentobarbital (Sigma, Deisenhofen, Germany) intraperitoneally and were supplemented with 20 mg/kg per hour pentobarbital intravenously during the experiment.
Twelve to fourteen days post-surgery, animals were initially anaesthetized with Ketamine (10 mg/kg, intramuscularly) and then deeply anaesthetized with pentobarbital (25 mg/kg, intravenously) and transcardially perfused with saline followed by a 4% paraformaldehyde and 1.5% sucrose solution in 0.1 m PB, pH 7.4.
The mice were anaesthetized as described [17].
The following morning, animals were anaesthetized as described above.
Before introduction into the swim-tunnels, fish (n = 167) were anaesthetized as described before and measured.
After 20 experimental days, fish were removed from the tunnels and were anaesthetized as described before, counted and measured again.
One hour following tMCAO, animals were anaesthetized as mentioned before, mounted on the animal holder of the scanner and the body temperature was kept maintained.
Mice were anaesthetized as described above and perfused by intracardial injection of 10 mL cold phosphate buffered saline and 10 mL 4% paraformaldehyde in phosphate buffered saline.
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