Despite the limited fidelity of the initial wound response, the wounded epithelium is able to establish a robust healing response.
Indeed, the CV for Ca2+ wound response shows statistically higher variability than ERK wound response indicating that paracrine communication reduces response variability during initial wound response.
Standard wound healing assays often deal with just one initial wound shape, and it is unclear whether varying the initial wound shape might impact how we interpret results from these experiments.
In treated horses, initial wound expansion was significantly decreased during the first 2 weeks.
The extent of wound closure was calculated as the percentage of the initial wound until final wound closure at 24 h time point using ImageJ software.
Measurements of wound area as a function of time show that the rate of wound closure depends on the initial wound shape.
Therefore, parameter estimates from an experiment with a particular wound shape can be used to predict an experiment with a different initial wound shape.
This is interesting because the only aspect of the assay we change is the initial wound shape, and the reason for different closure rates is unclear.
In a rat skin excisional model, the collagen-targeting glue successfully accelerated initial wound regeneration as defined by effective reepithelialization, neovascularization, and rapid collagen synthesis.
There was no difference between groups with regard to age, initial wound volume, type of surgical procedure, incision, or body mass index.
Head shots in that era were almost always fatal, but Bierce survived not only the initial wound, but an awful two-day train ride on an open flatcar to an army hospital in Chattanooga.
