Provided that the initial values are calculated, the cost of the rest calculation at step j is dominated by the number of the coefficients A (i, n j −1, n j + α ) for 0≤ i ≤ n j + α and 1≤ α ≤ N j +1 and it is O (N j 2 ).
The data was exported from the Multimode Analysis Software, and the initial rate values were calculated using Microsoft Excel.
Further, the initial and final GRAVY values were calculated by ProtParam for comparison.
Starting with initial concentrations, steady-state values were calculated under acyl-CoA dehydrogenase activity conditions of healthy controls.
Ki values were calculated from initial rates by the method described in Zhou et al. (1997).
Effect values were calculated based on nominal initial concentrations referring to soil dry mass.
The values were calculated relative to the initial value t0 that was set to 1.
The kapp values were calculated by dividing the initial velocity by the enzymic biotin concentration in each assay.
The average±S.E.M. values were calculated and transformed into initial copy number values for the purpose of displaying the error bars in graphical representations of the data.
EC50 values were calculated from plots of the initial rate of Ca2+ release as a function of BDE-4 concentration.
