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At the end, these nodes and their paths are discarded and the initial trimmed GO graph is reduced in size.
Once the "initial trimmed GO graph" has been obtained, each node is weighted according to the BLAST e-values.
In the "initial trimmed GO graph" some terms may belong to hits that are not functionally related to the original query.
Once GO hits and their corresponding scores are obtained, they are processed in order to reconstruct all of the possible paths leading to the root node; the rest of the GO nodes that does not belong to the reconstructed paths are discarded and we finally obtain the "initial trimmed GO graph" (see Fig. 1-i).
Raw reads were processed for initial trimming by CLC Genomics Workbench (version 5.5.2; CLC bio, Aarhus, Denmark).
For initial trimming experiments, we varied these quality thresholds as indicated below.
Initial trimming was performed using 0.5 as the limit of the quality score (based on Phred quality scores), and resulting quality of the trimmed reads was visualized with FastQC (http://www.bioinformatics.bbsrc.ac.uk/projects/fastqc/).bbsrc.ac.uk/projects/fastqc/
Initial trimming of the read data was performed using the CLC Genomics Workbench (CLC Bio) v.4.8 such that very short reads (<100 bases), terminal nucleotides (five from each end), low quality reads (quality limit 0.05), and 454 sequencing adapters and primers were removed from the dataset.
CW performed the initial SeqClean trimming.
The CLC Genomics Workbench CLC Bioo) v.4.8 was used for initial read trimming.
An initial vector trimming task revealed a design flaw with the SeqClean program.
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