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During initialization, the centroids of the planar segments that represent the checkerboard patterns in the laser scanner's point cloud are used to calculate the initial transformations.
Module 1 (data collection and processing module) performs automated data extraction and initial transformations.
In case no colonies could be obtained from the initial transformations, this procedure was repeated for two more times.
Only if the rescued and retransformed plasmids showed the same digestion pattern as the original plasmid, the rescue was counted as successful, In case no colonies could be obtained from the initial transformations, this procedure was repeated twice.
The reason behind that is the fact that the Structal method depends on the initial alignments for its initial transformations and it might miss the true alignment if the initial alignments are not good starting points.
To guide the initial transformations, 22 control points are manually placed as rough local landmarks for hippocampal segmentation: one point at the hippocampal head, one at the tail, and four per image (i.e. at the superior, inferior, medial and lateral boundaries) on five equally spaced image slices perpendicular to the long axis of the ipsilateral hippocampus.
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In order to reduce the arguably biologically implausibly large number of stages required by their first model, Armitage and Doll [ 105] developed a further model of carcinogenesis, which postulated a two-stage probabilistic process whereby a cell following an initial transformation into a pre-neoplastic state (initiation) was subject to a period of accelerated (exponential) growth.
With pAbr3, the SGFP signal not only increased in intensity and distribution, but most of the analyzed mycelial sections appeared to be enriched in transformed hyphae even after 1 year from the initial transformation.
The insert containing plasmid was recovered after initial transformation in E. coli BP5α cells (BioPioneer), confirmed by DNA sequencing and transformed into E. coli BL21 (DE3) cells (Novagen).
Additionally, studies with cervical tumors revealed that not only is E6 expressed immediately following the initial transformation, but expression is retained and can be detected long after the transforming event [ 17, 77].
Different clock-marker lines, CCR2::LUC, CCA1 LUC, LHY::LUC, TOC1 LUC, and GI::LUC, used in the study were generated by initial transformation of the respective marker into Bay-0, followed by crossing the T2 transformants to the target genotype: Bay-0, NIL-S, elf3-1, and elf3-4.
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