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And to be fair, it was actually Terry J who established the initial template for my contribution to the group that eventually became Monty Python.
Michael Hertz, whose firm is credited with designing the initial template for the map, has long chafed at Mr. Tauranac's calling himself the "design chief" on a project that has garnered numerous accolades, including a commendation from the United States Department of Transportation and the National Endowment of the Arts.
The Gal4-luciferase vector (pFRluc, Stratagene) was used as an initial template for construction of the reporter vector.
The initial template for GALA-LRRs was taken from a 24-residue LRR (residues 2211 to 2235) of the known crystal structure of human Skp2 protein [9], [10] (see Results and Discussion) using the Insight II program [35].
This provided an initial template for analysis which was subsequently revised to develop further sub-categories.
One hundred nanogram of double-stranded cDNA was used as initial template for restriction digestion with PstI and MseI (KeyGene).
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R-GECO1 and R-GECO1.2 in pTorPE [ 5] were used as the initial templates for engineering low-affinity red fluorescent genetically encoded Ca2+ indicators for optical imaging (LAR-GECOs).
The initial template of the model is an EM structure.
Three initial template volumes for ML3D classification were prepared by splitting the data into three random subsets.
To minimize head motions, motion correction (Jenkinson et al., 2002) was implemented, with the middle volume as an initial template image for registration.
Initial template concentrations for both species were quantified with NanoDrop 1000 Spectrophotometer (Thermo Scientific) and the appropriate dilutions were done so equal concentrations were used in the Q-PCR reactions.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com