Because of the presence of a tough pollen coat, major modifications were introduced in the initial steps consisting in squashing pollens between a microscope slide and a cover slip, retrieving them with 50 μl CTAB buffer and heating the solution for 15' at 95°C.
The initial PCR step consisted of a 10-min hold at 95 °C, followed by 40 cycles consisting of a 15-s denaturation at 95 °C and a 1-min annealing/extension at 63 °C.
The initial PCR step consisted of a 10 min hold at 95 °C, followed by 40 cycles consisting of 15 s denaturation at 95 °C and 1 min annealing/extension at 60 °C.
The initial PCR step consisted of a 10-min hold at 95°C, followed by 40 cycles of 15-s denaturation at 95°C, and 1 min annealing/extension at 63°C.
The initial step, presumably, consisted of insertion of a retrotransposed gene, followed by an increase in copy number and diversification of small RNA genes, which would not have been counter-selected at the loci involved.
The amplification step consisted of an initial denaturation at 95 °C, followed by 40 cycles of denaturation at 95 °C for 15 s and then annealing at 60 °C for 1 min.
The initial step of implementation consisted of an assessment of the information technology status at each HC and laboratory, performed by the regional health districts, and included data such as the number and condition of computers in each HC, physical security, and internet access.
The initial step of the pathway consisted of i) either AtoB from E. coli or NphT7 from Streptomyces sp. to convert the metabolic pathway intermediate, acetyl-CoA to acetoacetyl-CoA [ 17, 21].
