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As reported previously (Choi et al., 2008), GFP-paxillin was not seen initially at the tip (Fig. 2F initial spreading; supplementary material Fig. S4D; Movie 6), but appeared strongly with the onset of contractile spreading phase (Fig. 2F white dotted arrow; supplementary material Movie 6).
Initial spreading was defined as the first time point where contact was initiated and (i) contact was maintained for at least 45 s, and (ii) contact area reached a minimum level of 7.8 μm (corresponding to 500 pixels).
Also the avidin biotin binding system enhanced cell initial spreading.
Initial spreading velocities as fast as ~0.5 m/s have been recorded.
The second mode represents the equilibration of slopes within the beach fill and the initial spreading of the fill.
The operating conditions are chosen to exemplify the effect of the coaxial jet outer shear layer initial spreading rates.
A notable universal relationship has been proposed in the literature for the evolution of dimensionless droplet height and wetting diameter during the initial spreading stage of droplet impingement.
If a drop of oil is placed on a flat liquid substrate its behavior is determined by the initial spreading coefficient S: S = σwo + σgo.
They observed the inability of cells to adhere and subsequently spread on surfaces with ZnO nanorods, suggesting that the lack of initial spreading is probably the main cause of cell death rather than the long-term exposure to ZnO.
The number of layers plays a significant role only for DS-terminated films with Fn, where spreading on a bilayer greatly exceeds that on a multilayer, and PLL-terminated films without Fn, where initial spreading is significantly higher on a monolayer.
In contrast, a very thin crust with a minimum thickness of 3 4 km and a higher P n velocity of 8.1 8.3 km/s characterize the western end of the SB, which might have been formed during rifting and the initial spreading stages.
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