This divide and conquer approach thus shortcuts some of the anticipated problems faced during the initial rounds of selection.
That is, for the initial rounds of selection, high micromolar concentrations of hapten were used to capture poorly expressed phage antibodies that could evolve into high affinity binders.
For three additional ePK genes, cdpk7, tkl3, and PBANKA_130520, genotyping was reproducibly positive in a subpopulation of parasites after the initial round of drug selection, but dilution cloning failed, probably due to insufficient fitness of the mutants.
After an initial round of puzzlement over this selection, conservatives backed the nomination, even though Roberts never gave any solid indication of whether he agreed with the philosophy of judicial restraint.
Those efforts included an initial round of White House meetings.
The company is accepting applications for the initial round of grants through July 1.
The previous section clearly shows one round localized re-authentication, which also represents initial round of iterative process.
CIRM will likely award its final round of grants from its initial round of funding in 2017.
HT did the initial round of modeling and contributed part of the literature review.
Thus 86% of the products were successfully amplified during our initial round of two-stage PCR.
