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This method has serious drawbacks, primarily related to the subjectivity in estimating initial rates from experimental data.
Initial rates from steady-state assays using saturating αKG concentrations (500 μM), ambient O2 concentrations (217 μM), and varied concentrations of CTAD (15 300 μM) were fit to the Michaelis Menten equation.
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The reaction was first allowed to proceed to ≈25 % conversion to allow the determination of an initial rate from the linear reaction phase.
For each rate determination, a time course was run and linear regression was used to estimate the initial rate from early samples of the time courses, during which the rate visually appeared to be linear.
Initial rates (v) from the kinetic studies were plotted as 1/v vs. 1/[substrate concentration].
The initial rates recorded from the early regions of the progress curves were fitted against the Michaelis-Menten equation V = kcat [E]0 [S]/ KM + [S]) using KaleidaGraph 3.5 to determine the kinetic constants.
The initial rates obtained from these experiments were fit to eq 1.
After the exclusion, the immigrant death rates substantially increased compared to the initial rates calculated from the whole migrant population.
Steady-state kinetic parameters were determined from initial rates, which were calculated from the changes in phenylhydrazone absorbance at 540 nm.
Initial rates were obtained from linear regression of the data from early parts of the time courses, where the reactions visually appear to be linear.
Trehalose concentration (wv-1) Initial rate (nmoles mg-1min-1) 0 0.12 10 0.16 25 0.15 Satrehaloserehalose 1.15 Saturated K2S04 0.96 Initial rates were calculated from the aliquots taken over 0.5 to 3 h.
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