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These four parameters can be adjusted to generate the initial phasing model.
Our initial phasing model is a 14-Å cryo-EM HEV-VLP reconstructed from the Step 10 20.
For obtaining the phase information, cryo-EM model from the second step is used as an initial phasing model.
The initial phasing was carried out using Oasis [29] and Solve/Resolve [30] using the 2.9 Å Se-SAD data.
Initial phasing was performed by molecular replacement using the structure of the human Fab PDB entry 1AQK as template and using the software Phaser [21].
The Selenium positions were located by use of the program ShelxD and the initial phasing was carried out by using SOLVE [23].
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