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Some females carry a live mealybug with them on the mating flight and take it to the new colony site, where the mealybug's offspring provide the honeydew to feed the ant's initial offspring.
Genomic DNA was isolated from fin clips of 125 brooders and 552 offspring from the initial offspring panel (n = 2146), by standard proteinase-K digestion, following the salting out procedure as described in Miller et al. [50].
The initial offspring of these unions were of mixed ancestry, and their subsequent intermarriages gave rise to a new Aboriginal people called the Métis with a unique history, culture, and language.
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In our initial studies, offspring from Plp1tg carrier mice were identified following the procedure of Readhead et al. (1994) for the presence or absence of the T7 promoter sequence contained in the inserted vector.
To increase efficiency, the ISP (Ho and Ji 2003, 2004) shown in Fig. 3, is used to improve the links of each initial solution and each offspring generated by the three genetic operators.
From a proximate perspective, early hatching gives the first-laid offspring an initial competitive advantage, potentially enabling it to grow more rapidly than its younger siblings due to a positive feedback loop between competitive ability and resource acquisition.
Isofemale populations were immediately expanded to a large population size following the initial mating (approximately 100 1000 offspring per line in the first generation and very large population sizes in subsequent generations).
The initial fitness of an offspring individual is set to a predefined value F 0, its strength is drawn from a uniform distribution with minimum zero and maximum one and all values of the willingness to escalate w i,j are set to one.
The fact that, in yeasts, sexual reproduction results in bi-parental transmission of mitochondria argues in favour of the view that, when sex evolved in the ancestral diploid eukaryote, both parents probably contributed to the offspring's initial stocks of mitochondria.
Childhood is also proposed as a period of vulnerability, especially in the germline of females, since oocytes remain in a haploid de-methylated state until puberty, so environmental insults may potentially disturb the epigenetic state of the oocyte for many years (Faulk and Dolinoy 2011), with potential implications for both fertility and initial epigenetic state of offspring of an exposed female.
In an initial sample of parents and offspring of 57 full-sib families (N = 470 offspring), traits S1 and S2 had high heritabilities (0.54 ± 0.05; 0.64 ± 0.06 respectively) and additive genetic coefficients of variation typical of those found in fly wings (1.5% and 1.6%; c.f. [ 36]).
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