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The initial level of expression of investigated cDNA was determined by standard calibration curves (versus "standard" cDNA) and this value was obtained for each gene of interest and normalized according to the amount of the reference gene cDNA [ 43].
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Hif1α mRNA expression did not differ between the control and mutant mice although the initial level of Hif1α expression was higher in the WT livers than in the Gstp1/2 −/− livers.
Difference in the accumulation of progerin between different types of tissues may be dependent on the initial level of progerin expression, which varied between different tissue types, or due to the detection range for the progerin antibody being limited.
Variation in transgene expression in independent transgenic lines has been repeatedly reported to be related to the sequence of the introduced gene construct, involving RNA-sensing mechanism, the locus of insertion, the number of insertion copies, and the initial level of transgene expression [ 8- 13].
The presence of astrocytes reduced the initial level of radiation-induced γH2AX expression, indicative of a reduction in radiation-induced DSBs 12– 12.
The initial levels of phospholipase expression in the resistant variety at 2 DPI was approximately 1.3-fold higher than the susceptible one, and this further increased at 3 DPI and maintained this level even at 4 DPI.
Given the strong basal expression level of STAT3, we assume that the initial level of STAT3 protein is non-zero.
This is consistent with the findings of other groups and represents a transition from initial high levels of expression arising from unintegrated (episomal) pDNA to steady-state expression levels resulting from integrated pDNA[21], [24], [32].
Traditional filtration on a minimal level of expression and fold change will exclude these genes from initial examination.
Level of expression (general prominence) ('LoE').
Yellow, high level of expression; blue, low level.
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