Since few animals receiving no treatment or HS alone were alive for comparison after 48 h from Study 1 (Figure 1), analysis was conducted over the initial 48 h as lethality developed.
Initial experiments were focused on comparing lethalities of sodium hypochlorite (NaOCl) and ClO2.
Initial results showed a large variability of lethality in flour, obtaining between 0.2 and 3.0 log10 cycles of inactivation.
lethal dose with 50% lethality.
lethal concentration with 50% lethality.
This finding resonates strongly with the initial experiments on Rest−/− mice, which displayed embryonic lethality around E11, but importantly, showed no widespread precocious expression of Rest target genes.
Deletion of the gene encoding heparan sulfate GlcNAc N-deacetylase/N-sulfotransferase-1 (Ndst1), one of a family of four enzymes involved in the initial sulfation of the heparan sulfate chains, leads to perinatal lethality with lung, brain and skeletal defects [40], [41], [42].
Our initial expectation was that if the sterility was suppressed, then complete embryonic lethality would result because we know that wee-1.3 mutants are embryonic lethal and all the candidates tested were also reported to be embryonic lethal.
To determine the feasibility of developing an oat TILLING population initial experiments were performed to find an optimal balance between mutation frequency and lethality.
Our results suggest skin tissue inflammation is part of the initial response to first infection and may be an important factor in the lethality of Bd infection in susceptible individuals.
